7WM6
Crystal structure of SAH-bound TrmM from Mycoplasma capricolum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97942 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.390, 75.890, 95.540 |
Unit cell angles | 90.00, 95.18, 90.00 |
Refinement procedure
Resolution | 47.640 - 2.540 |
R-factor | 0.2121 |
Rwork | 0.209 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lpm |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.640 | 47.640 | 2.650 |
High resolution limit [Å] | 2.540 | 8.800 | 2.540 |
Rmerge | 0.215 | 0.065 | 2.385 |
Rmeas | 0.235 | 0.072 | 2.612 |
Rpim | 0.093 | 0.029 | 1.046 |
Total number of observations | 210998 | 4990 | 24841 |
Number of reflections | 34219 | 853 | 4161 |
<I/σ(I)> | 6.9 | 19.2 | 1.4 |
Completeness [%] | 97.5 | 95.8 | 96.9 |
Redundancy | 6.2 | 5.8 | 6 |
CC(1/2) | 0.992 | 0.994 | 0.413 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M HEPES pH 7.5 0.2 M Ammonium sulfate 25% w/v Polyethylene glycol 3,350 |