7WM6

Crystal structure of SAH-bound TrmM from Mycoplasma capricolum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97942
Spacegroup name	P 1 21 1
Unit cell lengths	74.390, 75.890, 95.540
Unit cell angles	90.00, 95.18, 90.00

Refinement procedure

Resolution	47.640 - 2.540
R-factor	0.2121
Rwork	0.209
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lpm
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.640	47.640	2.650
High resolution limit [Å]	2.540	8.800	2.540
Rmerge	0.215	0.065	2.385
Rmeas	0.235	0.072	2.612
Rpim	0.093	0.029	1.046
Total number of observations	210998	4990	24841
Number of reflections	34219	853	4161
<I/σ(I)>	6.9	19.2	1.4
Completeness [%]	97.5	95.8	96.9
Redundancy	6.2	5.8	6
CC(1/2)	0.992	0.994	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M HEPES pH 7.5 0.2 M Ammonium sulfate 25% w/v Polyethylene glycol 3,350