7WM5

Crystal structure of apo TrmM from Mycoplasma capricolum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	H 3 2
Unit cell lengths	112.710, 112.710, 110.178
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.510 - 2.150
R-factor	0.2051
Rwork	0.202
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lpm
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.190
High resolution limit [Å]	2.150	5.820	2.150
Rmerge	0.082	0.051	0.567
Rmeas	0.091	0.056	0.649
Rpim	0.039	0.022	0.305
Total number of observations	71295
Number of reflections	13888	687	674
<I/σ(I)>	5.8
Completeness [%]	93.3	86.2	91.8
Redundancy	5.1	5.9	3.8
CC(1/2)		0.997	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	2.0 M Sodium chloride 10% w/v Polyethylene glycol 6,000