7WLV
Crystal Structure of the Multidrug effulx transporter BpeF from Burkholderia pseudomallei.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 119.875, 121.141, 159.745 |
| Unit cell angles | 97.39, 111.19, 100.60 |
Refinement procedure
| Resolution | 49.020 - 3.000 |
| R-factor | 0.2133 |
| Rwork | 0.211 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dx5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.263 |
| Data reduction software | XDS |
| Phasing software | PHENIX ((1.20_4459: ???)) |
| Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.020 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.094 | 0.661 |
| Number of reflections | 160880 | 7513 |
| <I/σ(I)> | 8.5 | 0.9 |
| Completeness [%] | 99.5 | 93.07 |
| Redundancy | 5.3 | 1.84 |
| CC(1/2) | 0.997 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 298 | 50mM N-(2-Acetamido)iminodiacetic acid, 100mM Lithium Chloride, 200mM Ammonium Sulfate, 36% PEG 300 |
