7WLP
Epstein-Barr virus protein BKRF4 restricts nucleosome assembly to suppress host antiviral responses
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2019-05-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 88.270, 88.270, 105.626 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.250 - 2.290 |
R-factor | 0.2278 |
Rwork | 0.227 |
R-free | 0.24160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cv5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.509 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.290 | 2.290 |
Number of reflections | 11406 | 1077 |
<I/σ(I)> | 27.7 | 1.4 |
Completeness [%] | 99.7 | 97.7 |
Redundancy | 16.7 | 10.3 |
CC(1/2) | 1.000 | 0.830 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 2M Ammonium sulfate |