7WKR

Room temperature structure of lysozyme solved by serial synchrotron crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2019-02-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	78.530, 78.530, 38.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.530 - 1.600
R-factor	0.1746
Rwork	0.173
R-free	0.19840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dtb
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.360	1.650
High resolution limit [Å]	1.600	1.600
Number of reflections	16271	1600
<I/σ(I)>	7.21
Completeness [%]	100.0
Redundancy	290.2
CC(1/2)	0.991	0.419

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293.5	sodium acetate, PEG 8000, NaCl