7WKP
ICP1 Csy4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03923 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 57.892, 57.892, 355.786 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.040 - 2.000 |
R-factor | 0.2156 |
Rwork | 0.214 |
R-free | 0.24380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xli |
RMSD bond length | 0.002 |
RMSD bond angle | 0.524 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.040 | 2.067 |
High resolution limit [Å] | 1.996 | 1.996 |
Rmerge | 0.145 | 1.880 |
Number of reflections | 25046 | 2101 |
<I/σ(I)> | 26.314 | |
Completeness [%] | 98.4 | |
Redundancy | 25.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.4M Sodium thiocyanate, 0.1M Sodium acetate pH 4.0, 16% w/v PEG 4000 |