7WKO
ICP1 Csy1-2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.952, 67.758, 74.452 |
Unit cell angles | 90.00, 101.49, 90.00 |
Refinement procedure
Resolution | 36.480 - 2.300 |
R-factor | 0.1949 |
Rwork | 0.192 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7wwv |
RMSD bond length | 0.008 |
RMSD bond angle | 1.017 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.480 | 2.381 |
High resolution limit [Å] | 2.299 | 2.299 |
Rmerge | 0.094 | 1.277 |
Number of reflections | 17672 | 760 |
<I/σ(I)> | 19.633 | |
Completeness [%] | 81.0 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M BIS-TRIS propane pH 9.0, 20% v/v Polyethylene glycol monomethyl ether 550 |