7WKO
ICP1 Csy1-2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.952, 67.758, 74.452 |
| Unit cell angles | 90.00, 101.49, 90.00 |
Refinement procedure
| Resolution | 36.480 - 2.300 |
| R-factor | 0.1949 |
| Rwork | 0.192 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7wwv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.017 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.480 | 2.381 |
| High resolution limit [Å] | 2.299 | 2.299 |
| Rmerge | 0.094 | 1.277 |
| Number of reflections | 17672 | 760 |
| <I/σ(I)> | 19.633 | |
| Completeness [%] | 81.0 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M BIS-TRIS propane pH 9.0, 20% v/v Polyethylene glycol monomethyl ether 550 |
