7WJP

Structure of PadR-like protein from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-01
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 41 21 2
Unit cell lengths	72.182, 72.182, 46.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.090 - 2.250
R-factor	0.2282
Rwork	0.225
R-free	0.28850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zqh
RMSD bond length	0.007
RMSD bond angle	0.937
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.290
High resolution limit [Å]	2.250	2.250
Rmerge	0.073	0.387
Number of reflections	6256	188
<I/σ(I)>	56.6	3.25
Completeness [%]	99.9	99.9
Redundancy	13.5	12.9
CC(1/2)	1.000	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	1 M lithium chloride, 0.1 M MES, pH 6.5, 17% polyethylene glycol 6000