7WJA

Crystal structure of Lactococcus lactis subsp. cremoris GH31 alpha-1,3-glucosidase, P6322 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-27
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 63 2 2
Unit cell lengths	151.560, 151.560, 176.611
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.659 - 1.850
Rwork	0.165
R-free	0.18760
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.769
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.091	1.144
Number of reflections	101843	14635
<I/σ(I)>	24.7	3
Completeness [%]	100.0	100
Redundancy	19.7	18.6
CC(1/2)	1.000	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	14% PEG 3350, 400 mM ammonium citrate buffer (pH 7.0)