7WJ2
Crystal structure of HLA-C*1402 complexed with 8-mer HIV gag peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-15 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.655, 76.885, 62.053 |
Unit cell angles | 90.00, 119.54, 90.00 |
Refinement procedure
Resolution | 38.440 - 1.280 |
R-factor | 0.1568 |
Rwork | 0.156 |
R-free | 0.17810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w6a |
RMSD bond length | 0.008 |
RMSD bond angle | 1.059 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.300 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.056 | 0.384 |
Number of reflections | 96254 | 4687 |
<I/σ(I)> | 36.6 | |
Completeness [%] | 99.0 | |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 100 mM sodium acetate, 20 % PEG Smear Low |