7WIS
Catalytic intermediate structure of N381A mutant of copper amine oxidase from Arthrobacter globiformis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-09-07 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 191.934, 63.472, 158.100 |
Unit cell angles | 90.00, 117.30, 90.00 |
Refinement procedure
Resolution | 26.359 - 1.900 |
R-factor | 0.1643 |
Rwork | 0.163 |
R-free | 0.19670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.105 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 140.486 | 26.359 | 2.000 |
High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
Rmerge | 0.095 | 0.461 | |
Rmeas | 0.151 | 0.113 | 0.538 |
Rpim | 0.077 | 0.060 | 0.275 |
Total number of observations | 492849 | 15059 | 69549 |
Number of reflections | 130556 | 4210 | 18760 |
<I/σ(I)> | 6.8 | 15.7 | 2.5 |
Completeness [%] | 98.0 | 96.2 | 97.1 |
Redundancy | 3.8 | 3.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.8 | 289 | 25 mM HEPES 1.05 M potassium sodium tartrate |