7WIR

Holo form of N381A mutant of copper amine oxidase from Arthrobacter globiformis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-07-01
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	C 1 2 1
Unit cell lengths	192.967, 63.016, 158.013
Unit cell angles	90.00, 117.63, 90.00

Refinement procedure

Resolution	22.450 - 1.500
R-factor	0.162
Rwork	0.161
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.012
RMSD bond angle	1.504
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	139.991	22.451	1.580
High resolution limit [Å]	1.500	4.740	1.500
Rmerge		0.072	0.473
Rmeas	0.107	0.086	0.554
Rpim	0.056	0.047	0.288
Total number of observations	964630	30324	133818
Number of reflections	265999	8604	37966
<I/σ(I)>	8.7	16.6	2.5
Completeness [%]	99.0	97.8	97.1
Redundancy	3.6	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6.8	289	25 mM HEPES 1.05 M potassium sodium tartrate