7WIK

Crystal structure of oligoribonuclease of Mycobacterium smegmatis mc2 155

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-05-15
Detector	PSI JUNGFRAU 4M
Wavelength(s)	0.96863
Spacegroup name	P 21 21 21
Unit cell lengths	60.250, 97.260, 146.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.070 - 1.870
R-factor	0.1813
Rwork	0.179
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2igi
RMSD bond length	0.009
RMSD bond angle	1.461
Data reduction software	autoPROC (1.1.7)
Data scaling software	XSCALE (Jan 26, 2018)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.070	1.906
High resolution limit [Å]	1.870	1.874
Rmerge	0.056	0.694
Rmeas	0.070	0.794
Rpim	0.032	0.377
Number of reflections	71290	3483
<I/σ(I)>	13.5	2
Completeness [%]	99.5	99.8
Redundancy	4.6	4.3
CC(1/2)	0.999	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	298	0.1M Tris-HCl, pH8.4, 0.2M Lithium sulphate, 30% v/v PEG-4000