7WIA
The apo-form of THF-II C22G riboswitch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 66.420, 66.420, 93.409 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.130 - 3.220 |
R-factor | 0.1716 |
Rwork | 0.170 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7wi9 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.449 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.100 |
High resolution limit [Å] | 3.050 | 8.270 | 3.050 |
Rmerge | 0.152 | 0.104 | 0.954 |
Rmeas | 0.156 | ||
Total number of observations | 80579 | ||
Number of reflections | 4001 | 443 | 444 |
<I/σ(I)> | 6.6 | ||
Completeness [%] | 99.9 | 99.6 | 99.6 |
Redundancy | 9 | 7.4 | 7.4 |
CC(1/2) | 0.961 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20mM HEPES pH7.5, 20-25% PEG 3350 |