7WI7

Crystal structure of human MCM8/9 complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 21 3
Unit cell lengths	185.616, 185.616, 185.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.500 - 6.600
R-factor	0.3287
Rwork	0.321
R-free	0.39770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dp3 7dpd
RMSD bond length	0.002
RMSD bond angle	0.513
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.500	6.710
High resolution limit [Å]	6.600	6.600
Number of reflections	4153	418
<I/σ(I)>	39.8	4.1
Completeness [%]	97.8	100
Redundancy	13.7	14.3
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1M Bis-Tris propone, pH 6.0-7.5, 0.6M sodium citrate