7WHH
Crystal structure of SARS-CoV-2 omicron RBD and human ACE2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-25 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 1.07180 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 104.706, 104.706, 227.098 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.540 - 2.600 |
R-factor | 0.1936 |
Rwork | 0.192 |
R-free | 0.23050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6m0j |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.660 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.260 | 2.450 |
Number of reflections | 39525 | 2586 |
<I/σ(I)> | 11.3 | |
Completeness [%] | 99.8 | |
Redundancy | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M L-Proline, 0.1 M HEPES pH 7.5, 10% w/v Polyethylene glycol 3350 |