7WFX
EVAA-KlAte1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97960 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.720, 114.505, 159.469 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.510 - 1.950 |
R-factor | 0.1643 |
Rwork | 0.164 |
R-free | 0.20160 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.072 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.146 | 0.760 |
Rmeas | 0.163 | 0.893 |
Rpim | 0.071 | 0.461 |
Number of reflections | 93872 | 4641 |
<I/σ(I)> | 10.848 | 1.444 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 4.9 | 3.6 |
CC(1/2) | 0.985 | 0.551 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 120mM alcohols (Molecular dimensions), 100mM buffer system 2 pH 7.5 (Molecular dimensions), 37.5% (v/v) precipitant Mix 4 (Molecular dimensions) |