7WF8
Crystal structure of mouse SNX25 RGS domain in space group P212121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.690, 52.420, 136.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.990 - 1.350 |
| R-factor | 0.1649 |
| Rwork | 0.164 |
| R-free | 0.19130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zv4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.929 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.420 | 52.420 | 1.370 |
| High resolution limit [Å] | 1.350 | 7.390 | 1.350 |
| Rmerge | 0.069 | 0.045 | 0.852 |
| Rmeas | 0.073 | 0.047 | 0.917 |
| Rpim | 0.025 | 0.015 | 0.332 |
| Total number of observations | 553629 | 4269 | 24810 |
| Number of reflections | 68289 | 505 | 3342 |
| <I/σ(I)> | 13.5 | 40.2 | 2.1 |
| Completeness [%] | 99.9 | 99.9 | 99.9 |
| Redundancy | 8.1 | 8.5 | 7.4 |
| CC(1/2) | 0.999 | 0.998 | 0.763 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 277 | 0.1M Tris pH 8.5, 25% PEG 8000 |
