7WEZ
Crystal structure of RRM domain of Cyclophilin 33-like protein of Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-07-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.07 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.469, 40.802, 97.387 |
| Unit cell angles | 90.00, 115.57, 90.00 |
Refinement procedure
| Resolution | 45.250 - 1.900 |
| R-factor | 0.22543 |
| Rwork | 0.223 |
| R-free | 0.27606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mdf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.560 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.250 | 5.166 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | 0.573 |
| Rmeas | 0.059 | 0.690 |
| Rpim | 0.033 | 0.379 |
| Number of reflections | 24799 | 1229 |
| <I/σ(I)> | 14.4 | 2 |
| Completeness [%] | 97.4 | 99 |
| Redundancy | 3 | 3.1 |
| CC(1/2) | 0.999 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289.15 | 0.2 M magnesium chloride hexahydrate, 0.1 M Tris hydrochloride pH 8.5 and 30 % (w/v) PEG 4000 |
