7WEJ
Crystal structure of the mouse Wdr47 NTD.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.384, 75.762, 170.023 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.880 - 3.090 |
| R-factor | 0.2593 |
| Rwork | 0.256 |
| R-free | 0.29000 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.423 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (v1.18) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.872 | |
| Rmeas | 0.094 | 0.954 |
| Rpim | 0.035 | 0.378 |
| Number of reflections | 25839 | 2601 |
| <I/σ(I)> | 13.1 | 1.36 |
| Completeness [%] | 98.4 | 97.8 |
| Redundancy | 6.9 | 6 |
| CC(1/2) | 0.989 | 0.808 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 8% (v/v) Tacsimate pH 7.0, 20% (w/v) PEG 3350 |
