7WDM
Fungal immunomodulatory protein FIP-gmi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.987 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 96.585, 96.585, 80.303 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.040 - 2.123 |
R-factor | 0.2061 |
Rwork | 0.205 |
R-free | 0.21730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f3h |
Data reduction software | HKL-2000 (7.21) |
Data scaling software | HKL-2000 (7.21) |
Phasing software | PHASER (2.7.0) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.040 | 2.199 |
High resolution limit [Å] | 2.123 | 2.124 |
Rmerge | 0.117 | 0.915 |
Number of reflections | 10434 | 1006 |
<I/σ(I)> | 34.41 | |
Completeness [%] | 94.5 | |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293.15 | 0.1 M Na3Citrate (pH 5.6) 0.5 M NH4H2PO4 |