7WD4
Crystal structure of the Ilheus virus helicase: implications for enzyme function and drug design
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 289.15 |
Detector technology | PIXEL |
Collection date | 2020-11-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 112.213, 112.213, 76.459 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.510 - 1.750 |
R-factor | 0.1587 |
Rwork | 0.157 |
R-free | 0.19810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wv9 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.729 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.071 | 0.293 |
Number of reflections | 49792 | 49652 |
<I/σ(I)> | 43.3 | |
Completeness [%] | 99.9 | |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289.15 | 0.1 M tris hydrochloride, pH 8.5, 0.2 M ammonium sulfate |