7WBF

Crystal structure of lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	79.135, 79.135, 37.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.570 - 1.600
R-factor	0.1752
Rwork	0.172
R-free	0.20950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dtb
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	16048	767
<I/σ(I)>	36.24	4.15
Completeness [%]	100.0	100
Redundancy	25.3
CC(1/2)	0.997	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.5	293.15	sodium acetate, PEG 8000, NaCl