7WB2

Oxidase ChaP-D49L/Y109F mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	40.740, 61.720, 92.750
Unit cell angles	90.00, 91.25, 90.00

Refinement procedure

Resolution	29.281 - 1.800
R-factor	0.216
Rwork	0.213
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a4z
Data reduction software	AUTOMAR
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.281	1.950
High resolution limit [Å]	1.800	1.950
Number of reflections	30838	1892
<I/σ(I)>	8.9
Completeness [%]	73.4
Redundancy	2.4
CC(1/2)	0.995	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.01M Zinc Sulfate Heptahydrate, 0.1M MES monohydrate pH6.5, 25% v/v Polyethylene glycol monomethyl ether 550