7WAX

MurJ inward occluded form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	56.560, 72.440, 75.250
Unit cell angles	90.00, 94.96, 90.00

Refinement procedure

Resolution	37.480 - 2.350
R-factor	0.2122
Rwork	0.209
R-free	0.25400
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	1.359
Refinement software	PHENIX ((1.18rc7_3834: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.480	2.434
High resolution limit [Å]	2.350	2.350
Number of reflections	25372	2530
<I/σ(I)>	9.92
Completeness [%]	100.0
Redundancy	37.9
CC(1/2)	0.995	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293.15	MPD, MgCl2, Tris-HCl