7WAP
Mevo lectin mutant D134A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9799 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.810, 169.260, 160.940 |
Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
Resolution | 84.620 - 3.100 |
Rwork | 0.228 |
R-free | 0.27480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bsb |
RMSD bond length | 0.013 |
RMSD bond angle | 1.989 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.630 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.148 | |
Number of reflections | 118097 | 14028 |
<I/σ(I)> | 4.7 | 2.1 |
Completeness [%] | 91.8 | |
Redundancy | 2.8 | |
CC(1/2) | 0.970 | 0.929 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.6 | 295 | 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 20% v/v 2-Propanol, 20% w/v Polyethylene glycol 4000 |