7WAI
Glutamyl-tRNA synthetase from Plasmodium falciparum (PfERS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-04 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.460, 47.363, 94.449 |
Unit cell angles | 90.00, 109.10, 90.00 |
Refinement procedure
Resolution | 45.373 - 2.105 |
R-factor | 0.1966 |
Rwork | 0.194 |
R-free | 0.24130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nyl |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.15rc1_3423) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.400 | 2.142 |
High resolution limit [Å] | 2.105 | 2.105 |
Rmeas | 0.059 | 2.020 |
Number of reflections | 35766 | 1100 |
<I/σ(I)> | 15 | 0.6 |
Completeness [%] | 92.6 | |
Redundancy | 6.3 | |
CC(1/2) | 1.000 | 0.420 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Lithium sulfate, 0.1 M Tris pH 8.5, 30 % w/v PEG 4000 |