7WA3
Structure of American mink ACE2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-01 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.697, 97.725, 105.063 |
Unit cell angles | 90.00, 91.99, 90.00 |
Refinement procedure
Resolution | 42.190 - 2.280 |
R-factor | 0.2283 |
Rwork | 0.228 |
R-free | 0.24520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r42 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.568 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.540 | 2.400 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.142 | 0.591 |
Number of reflections | 61639 | 9035 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 98.2 | |
Redundancy | 5.7 | |
CC(1/2) | 0.992 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Potassium thiocyanate, 30% w/v PEG 2000 MME2 |