7W9U
Crystal Structure of Zn bound human Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-14 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98011 |
Spacegroup name | P 1 |
Unit cell lengths | 49.200, 52.450, 53.370 |
Unit cell angles | 90.02, 90.00, 90.00 |
Refinement procedure
Resolution | 49.200 - 2.163 |
Rwork | 0.215 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ow6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.646 |
Data reduction software | XDS (Version Jan 31, 2020) |
Data scaling software | Aimless (0.7.4) |
Phasing software | MoRDa (1.4.01) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.200 | 2.210 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.149 | 1.124 |
Number of reflections | 28719 | 1627 |
<I/σ(I)> | 6.8 | 0.8 |
Completeness [%] | 96.0 | |
Redundancy | 3.5 | |
CC(1/2) | 0.992 | 0.197 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 M Zinc acetate dihydrate, 20% w/v Polyethylene glycol 3,350 |