7W8W
DMSPP- and 5-Me-Trp-bound 6-dimethylallyl tryptophan synthase, IptA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 93.15 |
Detector technology | CCD |
Collection date | 2017-07-24 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.728, 44.878, 78.336 |
Unit cell angles | 90.00, 98.38, 90.00 |
Refinement procedure
Resolution | 49.200 - 1.800 |
R-factor | 0.1951 |
Rwork | 0.193 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7w8u |
RMSD bond length | 0.008 |
RMSD bond angle | 0.950 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.200 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 31974 | 3165 |
<I/σ(I)> | 9.35 | |
Completeness [%] | 99.7 | |
Redundancy | 3.8 | |
CC(1/2) | 0.992 | 0.207 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | HEPES pH7.0, Ammonium Sulfate |