7W8I
Crystal structure of Zn bound human Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-14 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98011 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.906, 53.099, 54.085 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.893 - 1.943 |
Rwork | 0.226 |
R-free | 0.26250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ow6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.585 |
Data reduction software | XDS (Version Jan 31, 2020) |
Data scaling software | Aimless (0.7.4) |
Phasing software | MoRDa (1.4.01) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.910 | 1.990 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.138 | 4.590 |
Number of reflections | 10806 | 646 |
<I/σ(I)> | 12.4 | |
Completeness [%] | 99.4 | |
Redundancy | 12.8 | |
CC(1/2) | 0.998 | 0.325 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 M Zinc acetate dihydrate, 20% w/v Polyethylene glycol 3,350 |