7W8F
Crystal structure of siderophore binding protein VatD from Vibrio vulnificus M2799 complexed with Desferal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 130 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-02-15 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.800, 57.890, 62.700 |
| Unit cell angles | 90.00, 95.02, 90.00 |
Refinement procedure
| Resolution | 26.103 - 1.900 |
| Rwork | 0.176 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1efd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.628 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.300 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.085 | 0.345 |
| Number of reflections | 20814 | 2172 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30%(w/v) PEG-3,350, 50mM MgCl2, 0.1M Bis-Tris pH6.5 |
