7W86

Crystal structure of the DYW domain of DYW1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.28242
Spacegroup name	P 1 21 1
Unit cell lengths	32.945, 45.165, 37.023
Unit cell angles	90.00, 98.04, 90.00

Refinement procedure

Resolution	36.660 - 1.800
R-factor	0.18029
Rwork	0.177
R-free	0.23365
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.524
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.170	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	10107	616
<I/σ(I)>	12.8
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)	0.998	0.625

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Hepes pH 7.5, 0.1 M Sodium Acetate, 0.2 M Lithium sulfate, 25%(w/v) PEG4000