7W82
Crystal structure of maize RDR2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | H 3 2 |
Unit cell lengths | 149.036, 149.036, 345.473 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.023 - 3.100 |
R-factor | 0.232 |
Rwork | 0.230 |
R-free | 0.27030 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.291 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
Rmerge | 0.124 | 0.036 | 0.894 |
Rmeas | 0.141 | 0.041 | 1.011 |
Rpim | 0.067 | 0.020 | 0.468 |
Total number of observations | 120266 | ||
Number of reflections | 27101 | 2831 | 2682 |
<I/σ(I)> | 3.7 | ||
Completeness [%] | 99.7 | 98.7 | 99.9 |
Redundancy | 4.4 | 4.2 | 4.6 |
CC(1/2) | 0.998 | 0.749 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.6M Sodium Citrate, 0.1M MES, pH 6.5 |