7W7I
Crystal Structure of shaft pilin PitB from pilus islet-2 of Streptococcus oralis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976254 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 200.629, 34.530, 207.516 |
| Unit cell angles | 90.00, 92.92, 90.00 |
Refinement procedure
| Resolution | 49.600 - 3.590 |
| R-factor | 0.322 |
| Rwork | 0.320 |
| R-free | 0.35800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7f7y |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.354 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.603 | 3.758 |
| High resolution limit [Å] | 3.591 | 3.591 |
| Rpim | 0.186 | 0.502 |
| Number of reflections | 19653 | 837 |
| <I/σ(I)> | 4 | |
| Completeness [%] | 84.3 | 88.7 |
| Completeness (spherical) [%] | 56.2 | |
| Completeness (ellipsoidal) [%] | 84.3 | |
| Redundancy | 3.1 | 2.9 |
| CC(1/2) | 0.956 | 0.696 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 295 | 100 mM Bis-Tris pH 8.0, 200 mM zinc acetate dihydrate, and 20% PEG smear medium |
