7W76

Crystal structure of the K. lactis Bre1 RBD in complex with Rad6, crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97846
Spacegroup name	P 61 2 2
Unit cell lengths	113.221, 113.221, 386.165
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.180 - 3.050
R-factor	0.2396
Rwork	0.237
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ayz
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.100
High resolution limit [Å]	3.050	8.270	3.050
Rmerge	0.135	0.073	1.160
Rmeas	0.138	0.074	1.191
Rpim	0.027	0.014	0.246
Number of reflections	58132	3396	2716
<I/σ(I)>	4.6
Completeness [%]	99.7	100	96.9
Redundancy	10.3	13.85	8.35
CC(1/2)		0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	sodium acetrate trithydrate, ammonium sulfate