7W76
Crystal structure of the K. lactis Bre1 RBD in complex with Rad6, crystal form II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97846 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 113.221, 113.221, 386.165 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.180 - 3.050 |
R-factor | 0.2396 |
Rwork | 0.237 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ayz |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.100 |
High resolution limit [Å] | 3.050 | 8.270 | 3.050 |
Rmerge | 0.135 | 0.073 | 1.160 |
Rmeas | 0.138 | 0.074 | 1.191 |
Rpim | 0.027 | 0.014 | 0.246 |
Number of reflections | 58132 | 3396 | 2716 |
<I/σ(I)> | 4.6 | ||
Completeness [%] | 99.7 | 100 | 96.9 |
Redundancy | 10.3 | 13.85 | 8.35 |
CC(1/2) | 0.999 | 0.876 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | sodium acetrate trithydrate, ammonium sulfate |