7W74
Crystal structure of DTG rhodopsin from Pseudomonas putida
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-20 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.080 |
Spacegroup name | P 63 |
Unit cell lengths | 65.565, 65.565, 219.363 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.850 - 2.840 |
R-factor | 0.2321 |
Rwork | 0.229 |
R-free | 0.25620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kfq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.575 |
Data reduction software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.850 | 44.850 | 3.010 |
High resolution limit [Å] | 2.840 | 8.390 | 2.840 |
Rmerge | 0.239 | 0.058 | 1.113 |
Rmeas | 0.251 | 0.057 | 1.119 |
Number of reflections | 12567 | 501 | 2007 |
<I/σ(I)> | 8.81 | 28.11 | 2.01 |
Completeness [%] | 99.9 | 98.4 | 100 |
Redundancy | 10.7 | 10.9 | 9.8 |
CC(1/2) | 0.997 | 0.999 | 0.747 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 4 | 296 | Magnesium chloride, Sodium chloride, Sodium citrate, PEG 300 |