7W5H
The structure of trichobrasilenol synthase TaTC6 in complex with FsPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | BRUKER METALJET |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2021-06-17 |
Detector | BRUKER PHOTON 100 |
Wavelength(s) | 1.34318 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.559, 98.567, 72.355 |
Unit cell angles | 90.00, 94.59, 90.00 |
Refinement procedure
Resolution | 31.230 - 2.050 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.24570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4okm |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.900 | 2.080 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.127 | 0.341 |
Number of reflections | 91204 | 2000 |
<I/σ(I)> | 7.1 | 2.4 |
Completeness [%] | 99.6 | 98.5 |
Redundancy | 4.1 | 3 |
CC(1/2) | 1.000 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 297 | 1.2 M Sodium malonate, pH 6.0 |