7W58
Crystal structure of acyl-carrier protein synthase from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-09-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5417 |
| Spacegroup name | H 3 |
| Unit cell lengths | 67.640, 67.640, 85.779 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.630 - 2.270 |
| R-factor | 0.1691 |
| Rwork | 0.167 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gwm |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.125 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 6746 | 180 |
| <I/σ(I)> | 21.44 | |
| Completeness [%] | 97.5 | |
| Redundancy | 8.3 | |
| CC(1/2) | 0.990 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium acetate trihydrate, PEG 3350 |
