7W54

Crystal structure of a bacterial OTU DUB with Ub-PA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	73.650, 126.090, 207.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.045 - 2.640
R-factor	0.2178
Rwork	0.216
R-free	0.25320
Structure solution method	SAD
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.030	2.710
High resolution limit [Å]	2.640	2.640
Rmerge	0.102
Number of reflections	56934	3288
<I/σ(I)>	2.3
Completeness [%]	99.1
Redundancy	13.6
CC(1/2)	0.999	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	299	100 mM Magnesium formate, 15% (w/v) PEG 3350, pH 8.0