7W4Z

Crystal structure of fragmin domain-1 in complex with actin (AMPPNP-form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	95
Detector technology	CCD
Collection date	2017-06-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.12
Spacegroup name	P 21 21 21
Unit cell lengths	57.090, 91.070, 114.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.350 - 1.150
R-factor	0.1436
Rwork	0.143
R-free	0.15750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3j8a
Data reduction software	XDS (Mar 15,2019)
Data scaling software	XSCALE (Mar 15,2019)
Phasing software	MOLREP (11.5.02)
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.350	29.350	1.180
High resolution limit [Å]	1.150	5.140	1.150
Rmerge	0.068	0.046	1.017
Rmeas	0.072	0.048	1.106
Total number of observations	1562876
Number of reflections	209417	2606	14871
<I/σ(I)>	12.64	48.7	1.82
Completeness [%]	98.7	99.7	95.5
Redundancy	7.463	10.412	6.39
CC(1/2)	0.999	0.998	0.631

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM Na2HPO4, 19% PEG3350