7W4U
Structure of the M. tuberculosis HtrA S407A mutant at room-temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2020-11-24 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | H 3 |
Unit cell lengths | 108.620, 108.620, 62.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.670 - 2.300 |
R-factor | 0.1967 |
Rwork | 0.189 |
R-free | 0.20690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ieo |
RMSD bond length | 0.007 |
RMSD bond angle | 0.991 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.670 | 2.383 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 11713 | 1222 |
<I/σ(I)> | 3.9 | |
Completeness [%] | 94.9 | |
Redundancy | 3.4 | |
CC(1/2) | 0.974 | 0.324 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 298 | 0.1M HEPES, 1.4M Sodium Citrate tribasic dihydrate pH 7.5 |