7W43
Crystal structure of Bacillus subtilis YjoB N-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.333, 84.176, 180.222 |
Unit cell angles | 90.00, 112.29, 90.00 |
Refinement procedure
Resolution | 49.360 - 3.000 |
R-factor | 0.2393 |
Rwork | 0.237 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7w42 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.121 | 0.710 |
Number of reflections | 39002 | 1935 |
<I/σ(I)> | 11.4 | 1.5 |
Completeness [%] | 96.9 | |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8.5 | 293 | 30% (w/v) isopropanol, 0.1 M Tris-HCl, 30% (w/v) PEG 3350 |