7W3U

USP34 catalytic domain in complex with UbPA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-26
Detector	PSI PILATUS 6M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 2
Unit cell lengths	192.020, 71.330, 111.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.596 - 3.130
R-factor	0.2187
Rwork	0.217
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7w3r
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.596	3.242
High resolution limit [Å]	3.130	3.130
Rmerge	0.140
Number of reflections	27763
<I/σ(I)>	11.5
Completeness [%]	99.6
Redundancy	15.21
CC(1/2)	0.998	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	15% w/v PEG 3350, 0.8M Magnesium chloride hexahydrate, 0.6mM Lyso PG, 1 mM Facade R-EPC