7W3R
USP34 catalytic domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.120, 128.060, 64.400 |
| Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
| Resolution | 25.014 - 1.920 |
| R-factor | 0.2277 |
| Rwork | 0.226 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wch |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.470 | 1.989 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.048 | |
| Number of reflections | 60503 | 60396 |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 98.1 | |
| Redundancy | 14.83 | |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 16% w/v PEG 3350, 0.16M sodium bromide, 0.125M Magnesium formate dihydrate, 0.025M BIS-TRIS |
