7W3N
Crystal structure of Ufm1 fused to UFBP1 UFIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-04 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.411, 53.411, 82.231 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.414 - 1.600 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hkh |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.426 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.414 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.034 | 0.478 |
| Number of reflections | 18508 | 1850 |
| <I/σ(I)> | 34.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 28% 2-propanol, 3% PEG 200, 0.1M MES at pH 6.0 |
