7W34
The crystal structure of human CtsL in complex with 14b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-20 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.979183 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 109.361, 109.361, 92.481 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.690 - 2.890 |
R-factor | 0.2535 |
Rwork | 0.248 |
R-free | 0.30280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f06 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.690 | 39.690 | 2.970 |
High resolution limit [Å] | 2.890 | 12.940 | 2.890 |
Rmerge | 0.570 | 0.068 | 4.741 |
Rmeas | 0.581 | 0.070 | 4.838 |
Total number of observations | 169661 | ||
Number of reflections | 6525 | 92 | 455 |
<I/σ(I)> | 10.08 | 31.39 | 1.5 |
Completeness [%] | 99.9 | 96.8 | 98.9 |
Redundancy | 26.002 | 17.576 | 25.374 |
CC(1/2) | 0.994 | 0.999 | 0.520 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 289.15 | 100mM sodium acetate (pH 4.1), 15% (w/v) PEG 2000, protein concentration 8mg/ml |