7W2Q
Crystal structure of Pseudomonas aeruginosa transcriptional regulator PvrA in the narrow conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 |
Unit cell lengths | 44.162, 44.162, 251.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.080 - 1.800 |
R-factor | 0.1852 |
Rwork | 0.184 |
R-free | 0.21420 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 2.048 |
Data reduction software | HKL-3000 |
Data scaling software | XDS |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.080 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 44339 | 4425 |
<I/σ(I)> | 27.82 | |
Completeness [%] | 99.9 | |
Redundancy | 12.2 | |
CC(1/2) | 0.999 | 0.952 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 0.1M MES,14% PEG 4000 |