7W2P
Tudor domain of SMN in complex with a small molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 35.893, 35.893, 92.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.440 - 1.150 |
| R-factor | 0.1288 |
| Rwork | 0.128 |
| R-free | 0.14960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qq6 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.909 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Number of reflections | 22319 | 1064 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.4 | |
| CC(1/2) | 0.999 | 0.785 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 1.8 M sodium acetate, pH 7.0, 0.1 M Bis-Tris propane |
