7W2O
Crystal structure of Pseudomonas aeruginosa transcriptional regulator PvrA in the wide conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 84.098, 84.098, 148.378 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.030 - 3.210 |
R-factor | 0.2724 |
Rwork | 0.268 |
R-free | 0.31360 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.871 |
Data reduction software | HKL-3000 |
Data scaling software | XDS |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.030 | 3.300 |
High resolution limit [Å] | 3.210 | 3.210 |
Number of reflections | 9186 | 864 |
<I/σ(I)> | 41.11 | |
Completeness [%] | 99.2 | |
Redundancy | 18.2 | |
CC(1/2) | 0.993 | 0.851 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.2M ammonium sulfate,0.1M sodium cacodylate,30%PEG8000 |